Hossein Mortazavi; Arileza Mortazavi; Anahita Rostami; Mohammadraza Javadi; Robabeh Abedini; Amir Teimourpour; Kheirolah Gholami; Ali Khamesipour
Abstract
Background: Little data on severe cutaneous adverse drug reactions (SCADRs) is available, especially in Iran. Therefore, there is a need for more studies in this field. We aimed to evaluate the clinical pictures and laboratory data of patients with SCADR in a tertiary dermatology center in Tehran, Iran.Methods: ...
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Background: Little data on severe cutaneous adverse drug reactions (SCADRs) is available, especially in Iran. Therefore, there is a need for more studies in this field. We aimed to evaluate the clinical pictures and laboratory data of patients with SCADR in a tertiary dermatology center in Tehran, Iran.Methods: In this retrospective study, patients with a clinical diagnosis of SCADR based on the World Health Organisation’s definition and histopathologic findings were included. Causality and preventability measures were assessed based on previous criteria, including the Naranjo score and the Schomock and Thronton scale.Results: Thirty-nine patients with a mean age of 43 ± 17 years participated in the study. SCADRs were more common in females than in males (2.9/1). SCADRs included Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN), acute generalized exanthematous pustulosis (AGEP), and drug reactions with eosinophilia and systemic symptoms (DRESS). Thirty-one patients presented a Naranjo score of 5-8, indicating probable drug reactions. The remaining eight patients (with scores of 1-4) were determined as having possible drug eruptions. Regarding the category of culprit drugs, anticonvulsants (49%), antimalarials (15%), antibiotics (13%), and antihypertensives (10%) were themost frequent causes of SCADR, with lamotrigine being the single most common agent.Conclusion: The most frequent clinical presentation of SCADR was SJS/TEN, followed by AGEP and DRESS. The most frequent cause of SCADR was anticonvulsant drugs.
Nasrin Hamidizadeh; Behrooz Barikbin; Maryam Yousefi; Abbas Sahraei; Ali Khamesipour; Shima Younespour; Hanif Sadeghitehrani
Volume 14, Issue 2 , 2011, , Pages 48-51
Abstract
Introduction: Cutaneous Leishmaniasis (CL) is a parasitic disease caused by Leishmania species. Currently accessible treatments remain insufficient, and there is pressure to develop suitable and effectual options. In this study, we used different concentrations of podophyllin in vitro on leishmania parasites ...
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Introduction: Cutaneous Leishmaniasis (CL) is a parasitic disease caused by Leishmania species. Currently accessible treatments remain insufficient, and there is pressure to develop suitable and effectual options. In this study, we used different concentrations of podophyllin in vitro on leishmania parasites and then on leishmaniasis lesions in mice and compared their efficacy. Method: We used podophyllin (14.3 µg/ml) in vitro against leishmania major parasites, then in experimental animals in different concentrations. Results: Podophyllin (14.3 µg/ml) that used in vitro eradicated leishmania major parasites, but, in mice after four weeks was not effective and the diameter of the lesions increase with use of topical podophyllin. Conclusion: Despite the lethal effect on leishmania in vitro, treatment with different doses of podophyllin could not accelerate the healing process of the leishmaniasis lesions of the experimental rats.
Yahya Sohrabi; Mahmoud Reza Jaafari; Ali Badee; Seyed Hossein Hejazi; Seyed Ebrahim Eskandari; Akram Miramin Mohammadi; Ali Khamesipour
Volume 10, Issue 1 , 2007, , Pages 37-53
Abstract
Background and aim: Efficacy of vaccines is mainly depending on the type of adjuvant used. Efficacy trials of first generation vaccine against leishmaniasis showed a limited efficacy due to lack of an appropriate adjuvant. The objective of this study was to investigate whether positively charged liposomes ...
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Background and aim: Efficacy of vaccines is mainly depending on the type of adjuvant used. Efficacy trials of first generation vaccine against leishmaniasis showed a limited efficacy due to lack of an appropriate adjuvant. The objective of this study was to investigate whether positively charged liposomes containing ALM could induce Th1 type immune response in susceptible Balb/c mice.Materials and methods: Liposomes containing ALM were prepared by dehydration-rehydration method. Female mice, 6-8 weeks old were immunized subcutaneously with prepared liposomes or as control with empty liposomes, ALM alone or PBS, 3 times, 3 weeks apart. Different groups of mice were challenged with virulent L. major. Immune responses of animals were evaluated in vivo by delayed type hypersensivity (DTH) and in vitro by titration of anti-Leishmania antibody isotypes using ELISA technique.Results: The results showed that footpad swelling in the group of mice immunized with DRV-PC/CHOL-ALM-DDAB was significantly smaller than the control groups (p<0.05). IgG2a titer was significantly higher in immunized mice with DRV-PC/CHOL-ALM-DDAB compared the control groups (p<0.001) and DRV-PC/CHOL-ALM (p<0.05). DTH response of groups received ALM incorporated into liposomes were significantly stronger than the control groups (p<0.001).Conclusion: Smaller lesion size, stronger DTH response and a high IgG2a titer are indicative of a Th1 response. It seems that positively charged liposomes might be used as an immunoadjuvant to induce a Th1 type of response.
