Mohammad Dehghan; Sepideh Hajian; Nazila Alborzi; Afsaneh Borgheyee; Amir Hussein Noohi
Volume 12, Issue 1 , 2009, , Pages 13-15
Abstract
Background: Dermatophytes are a group of closely related fungi that invade keratinized tissues (skin, hair and nails) of humans and animals and produce infections called dermatophytosis. Our objective was to determine causative agents of dermatophytosis in Gorgan, North of Iran. Methods: Data was based ...
Read More
Background: Dermatophytes are a group of closely related fungi that invade keratinized tissues (skin, hair and nails) of humans and animals and produce infections called dermatophytosis. Our objective was to determine causative agents of dermatophytosis in Gorgan, North of Iran. Methods: Data was based on collecting specimens from 1108 patients clinically suspected to have fungal infection during five years from 2003 through 2007. Specimens were collected from hair, nail and skin and were investigated by direct examination and cultured in Sabouraud dextrose agar. Fungal colonies were identified by macroscopic and microscopic examination and supplementary tests. Results: 351 samples out of 1108 were positive for dermatophytes and 277 ones had positive cultures. Epidemophyton floccosum was the most frequent species (70.4%) followed by Trichophyton rubrum (14.5%) and Microsporum audouinii (7.2%). Regarding the location of the lesions, groin and nails were the most frequent sites that developed dermatophytosis in the majority of the patients. Conclusion: Dermatophytosis is probably still one of the most infectious diseases in Iran. The anthropophilic (E.fluoccosum) and zoophilic (T. rubrum) species were the most common causes of dermatophyte of tinea in Gorgan, north of Iran. The frequency of tinea was higher in females and tinea cruris showed a remarkably increasing rate and can be an important public health issue in Gorgan.
Rad Mahnaz Mahmoudi; Mohammadi Akram Mir Amin; Barton Richard C
Volume 11, Issue 1 , 2008, , Pages 17-20
Abstract
Background: Trichophyton rubrum (T. rubrum) is the most common cause of dermatophytosis of skin and nail tissue. Strain identification in Trichophyton rubrum is important for identification of strain-related differences in infectivity potential or transmissibility and epidemiological studies. PCR typing ...
Read More
Background: Trichophyton rubrum (T. rubrum) is the most common cause of dermatophytosis of skin and nail tissue. Strain identification in Trichophyton rubrum is important for identification of strain-related differences in infectivity potential or transmissibility and epidemiological studies. PCR typing could determine whether the original isolate is responsible for re-infection or a new strain has been acquired.Methods: A minipreparation method for DNA from dermatophytes was used. Tandemly repetitive subelements (TRS-1 & TRS-2) of NTS region at ribosomal DNA of 23 T.rubrum isolates were amplified and the PCR products were separated by electrophoresis in 2% agarose gel (200 mA, 140 V), visualized by staining with ethidium bromide, and photographed.Results: On the basis of copy number of TRS-1 and TRS-2, 8 out of our 23 samples were type 2 & II, respectively. Six of them were type 3 & II, four isolates were type 1 & II, two isolates were type 4 & II, two isolates were type 1 & I and one isolate was type 5 & II.Conclusion: In this study, most of T. rubrum isolates were type 2 & II, dissimilar to European studies where type 1 & II has been the most common. The present study showed that 26.1% of Iranian isolates were type 1 in contrast with a previous study which has demonstrated a much lower prevalence in Asians (5%).
N Alizadeh; Sh Sadr Ashkevary; J Golchai; A Maboodi; AA Falahati
Volume 7, Issue 4 , 2004, , Pages 255-260
Abstract
Background: In spite of prevention and control programs, dermatophyte infections have a high prevalence worldwide. Objective: To do a descriptive study of dermatophytosis in Guilan. Patients and Methods: 217 patients whom were visited in Razi Hospital in Rasht in 1381 were screened for dermatophytosis ...
Read More
Background: In spite of prevention and control programs, dermatophyte infections have a high prevalence worldwide. Objective: To do a descriptive study of dermatophytosis in Guilan. Patients and Methods: 217 patients whom were visited in Razi Hospital in Rasht in 1381 were screened for dermatophytosis and if had suspicious lesions, direct smear with KOH and culture were done. Results: 93 patients had positive smear (KOH 10%) and culture for dermatophytes. 50 patients were women and 43 patients were men. The most common patterns of dermatophytosis were tinea cruris (47.2%), tinea pedis (14%), tinea capitis (12.9%), onychomycosis, tinea manum, and tinea facei. Trichophyton mentagrophytes was the most common causative agent of tinea cruris that occurred more significantly in hot seasons. Conclusion: Tinea cruris was the most common type of dermatophytosis in this study. High prevalence of tinea cruris specially in women in comparison to the previous studies, may be due to occupational activity (Agriculture) and clothing of women in north of Iran.
A Zamanian; M Farshchian; H Nazeri
Volume 6, Issue 4 , 2003, , Pages 31-33
Abstract
Background: Tinea pedis is probably the most common form of dermatophytosis in developed countries and it is one of the public health problems in the most societies. Moreover, wearing shoes, sweating and maceration are its predisposing factors. Objective: To evaluate the prevalence and determination ...
Read More
Background: Tinea pedis is probably the most common form of dermatophytosis in developed countries and it is one of the public health problems in the most societies. Moreover, wearing shoes, sweating and maceration are its predisposing factors. Objective: To evaluate the prevalence and determination of type of tinea pedis in the personnel of Hamedan Sina Hospital. Patients and Methods: In a descriptive cross-sectional study, 156 employees of Hamedan Sina Hospital were examined clinically and by fungal skin smears. Results: Ninety-three (59.6%) of patients were male and 63 (40.4%) were female. Seven patients (4.48%) suffered from tinea pedis and T.mentagrophytis (Interdigital) was the main cause of infection. Conclusion: The prevalence of tinea pedis in this study was 4.48%, which is lower than its prevalence in developed countries.