Mohamad Afshar; Mahmoudreza Jafari; Mohamadmehdi Hasanzadeh Taheri; Mohsen Khorashadizadeh; Hamide Taheri Olyayie
Abstract
Background: Curcumin (diferuloylmethane) is one of the mostactive components of turmeric. This herbal compound has antiinflammatoryand positive wound-healing impacts. The principalobjective of this study was to evaluate the impacts of curcuminnanoliposomes on cell viability and motility of mouse fibroblastNIH ...
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Background: Curcumin (diferuloylmethane) is one of the mostactive components of turmeric. This herbal compound has antiinflammatoryand positive wound-healing impacts. The principalobjective of this study was to evaluate the impacts of curcuminnanoliposomes on cell viability and motility of mouse fibroblastNIH 3T3 cells and its wound healing effects on second-degreeskin burns in BALB/c mice.Methods: Mature male BALB/c mice (n = 48) were dividedinto 4 groups (n = 12 per group). Group one received curcuminnanoliposome ointment; the positive and negative control groups(groups 2&3) were treated with silver sulfadiazine and placebo,respectively, and group four (sham) received no treatment. Theburn wound was created by a metal device with a diameter of 1cm. Animals received treatment twice daily. On days 4, 7, 10, and14, deep anesthesia and a biopsy of the wound were performed,and a microscopic study and MTT assay were carried out.Results: Cellular studies on mouse fibroblast NIH-3T3 cellsshowed that low-dose curcumin nanoliposomes increased cellproliferation and motility at 8, 12, and 24 hours in comparisonwith the control group. In tissue samples of mice treated withcurcumin nanoliposome (day 14), less inflammation was observed,while granulation tissue formation, fibroblast proliferation,epithelialization, and collagen fiber synthesis increased significantlycompared with the control groups.Conclusion: Our study indicates the positive effects of curcuminnanoliposomes on the motility process of mouse fibroblast NIH-3T3 cells (in vitro) and on the inflammatory and proliferativephases (in vivo) of burn wound healing in mice.
Dyah Ayu Mira Oktarina; Roihan Mohamad Iqbal
Abstract
Background: Simvastatin is a beta-hydroxy-beta-methylglutaryl- CoA (HMG-CoA) inhibitor molecule with several pleiotropic (immunomodulatory, anti-inflammatory, and antioxidant) activities. In this study, we evaluated the protective effect of simvastatin on ultraviolet B (UVB)-induced photoaging of normal ...
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Background: Simvastatin is a beta-hydroxy-beta-methylglutaryl- CoA (HMG-CoA) inhibitor molecule with several pleiotropic (immunomodulatory, anti-inflammatory, and antioxidant) activities. In this study, we evaluated the protective effect of simvastatin on ultraviolet B (UVB)-induced photoaging of normal human dermal fibroblast cultures by assessing fibroblast proliferation, collagen deposition, and fibroblast morphology.Methods: This study was an in vitro experiment using normal human skin fibroblast cell cultures. Fibroblasts were then cultured and observations were made of fibroblast proliferation, collagen deposition, and cell morphology using various concentrations of simvastatin (0 nM, 0.01 nM, 0.1 nM, 0.5 nM, 1 nM, and 5 nM) and UVB exposure (100 mJ/cm2).Results: After UVB exposure, a significant decrease in fibroblast proliferation and collagen deposition was observed. Cells appeared thinner, and fibroblasts were less organized and more pointed. Simvastatin with 0.01 nM, 0.1 nM, 0.5 nM, 1 nM, and 5 nM levels could significantly maintain cell proliferation and collagen deposition compared to UVB-irradiated cell groups without simvastatin. Interestingly, fibroblast proliferation and collagen deposition in the simvastatin group above 0.5 nM were not significantly different from the normal human dermal fibroblast group. An increased level of collagen deposition was also confirmed by observing the fibroblast morphology, which had more red-smeared cells on Sirius red staining. The antioxidant activity of simvastatin might play a role in fibroblast proliferation and collagen deposition, protecting against UVB by inhibiting reactive oxygen species. Simvastatinmaintained fibroblast morphology, possibly by preventing DNA damage and maintaining membrane-bound collagen fiber deposition.Conclusions: Our findings revealed that simvastatin pretreatment mitigated UVB-induced photoaging in human dermal fibroblast cells by maintaining fibroblast proliferation, collagen deposition, and fibroblast morphology.
Moravvej Hamideh; Rad Mahnaz Mahmoudi; Toossi Parviz; Khorasani Mohammad Taghi; Mirzadeh Hamid
Volume 12, Issue 4 , 2009, , Pages 111-116
Abstract
Background: Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelialmesenchymal interactions, secreting various growth factors and cytokines that have a direct effect on epidermal proliferation, differentiation and formation of extracellular ...
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Background: Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelialmesenchymal interactions, secreting various growth factors and cytokines that have a direct effect on epidermal proliferation, differentiation and formation of extracellular matrix. They have been incorporated into various tissue-engineered and used for a variety of clinical applications, including the treatment of burns, chronic venous ulcers and several other clinical applications in dermatology and plastic surgery. Method: Isolated fibroblasts by the enzymatic process from foreskin were cultivated successively in a culture medium to establish cell banking. Foreskin and the last subcultured cells were checked for HBV, HCV, HIV, HSV I, HSV II, HTLV I, HTLV II, EBV, CMV, Treponema Pallidum, Mycoplasma sp. and Clamydia. The 1st, 5th and 10th subcultured cells were processed for immunocytochemistry studies using a panel of monoclonal antibodies including antibodies to MHC class I & II antigens for ensuring the elimination of superficial cell antigens during cultivation. Subcultured cells were karyotyped to find any chromosomal abnormalities. The best passages were chosen for culturing on silicone sheets provided by the Iran Polymer and Petrochemical Institute. Results: Evaluation for bacteria and viruses by molecular methods was negative. Karyotyping of cultured fibroblasts after the 10th passage showed some abnormalities. HLA expression was imperceptible in the cells obtained from the 10th sub-culture. The best passages were from 5th to 10th for banking and culturing on silicone sheets. Conclusion: Expression of HLA on fibroblast surfaces was diminished during subculturing. To prevent chromosomal abnormalities in fibroblast passaging, we should select the best colony that is expected to be chromosomally stable with the least antigenicity. In our study, the 5th to 10th sub-cultures were the best cells for the purpose of grafting and acceleration of the wound healing.
Moravvej Hamideh; Rad Mahnaz Mahmoodi; Zali Hakimeh; Nabai Leila; Toossi Parviz
Volume 12, Issue 1 , 2009, , Pages 4-8
Abstract
Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic ...
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Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to accomplish multiple goals including skin repair in defects such as burns and ulcers and also performing various research projects on these cells in order to further study of the mechanisms involved in wound healing, rejuvenation and medication effects. Method: We initially developed primary cultures of skin fibroblasts in a DMEM medium. These primary cultures were formed by washing and trypsinizing foreskin specimens followed by separation of epidermis from dermis and cutting the dermis into small pieces. In about 10 days, a monolayer of fibroblasts was formed. Result: We were able to develop the fibroblast bank successfully and to initiate other projects utilizing this bank. Conclusions: With these cultured cells, we would be able to perform different research projects including studying the mechanisms of wound healing, rejuvenation, drug affects, inflammatory mediators, growth factors, etc. Moreover, further progress in this field will result in our independence from requesting these cells from external sources.
J Movaffagh; MR Jafari; MH Amouzegar; SA Tabatabaei
Volume 9, Issue 1 , 2006, , Pages 2-16