M Bayat; M.Gh Gol Mohammadi; FS Rezaei
Volume 8, Issue 6 , 2006, , Pages 475-481
Abstract
Background and aim: Low-power lasers relief pain in some musculoskeletal disorders and accelerate wound healing process. However, there are few reports on effects of low-power lasers on mast cells. In this study the effects of low-power gallium aluminium arsenide laser (Ga.Al.As.laser) radiation on number ...
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Background and aim: Low-power lasers relief pain in some musculoskeletal disorders and accelerate wound healing process. However, there are few reports on effects of low-power lasers on mast cells. In this study the effects of low-power gallium aluminium arsenide laser (Ga.Al.As.laser) radiation on number and degranulation of mast cells of open skin wound bed of rats were studied using quantitative histological methods.Materials and Methods: Forty-six male rats were randomly divided into experimental and control groups. Each group divided into 3 subgroups based on duration of study: 4 , 7 and 15 days. Under general anesthesia and sterile conditions one circular full thickness skin wound was made on the doesum of neck of each rat. The wounding day was considered as the day zero. From the day one, experimental rats received Ga. Al. Ar. laser radiation at a energy density of 1.2 J/cm2. After doing daily treatments, on days 4, 7 and 15, rats were killed by ether and samples were obtained from wound bed and normal adjacent skin from each rat. Samples were fixed in formalin saline and were prepared for routine histological study. Sections were stained by 0.1% watery solution of toluidine blue and total number of mast cells and their grades (one, two and three) were counted. In grade one, mast cell is intact, in grade two some granules have been extruded from the cell and in the mast cells of grade 3, degranultion is more extensive and widespread. Data were analysed by Student t test.Results: On the day 4, total number and grade one mast cells were significantly higher in the control group (P<0.01). On days 7 and 15, total number of mast cells and their grades were higher in the experimental group. The grade 2 mast cells on day 7 and grade 1 mast cells on day 15 of experimental group was significantly higher than control group (P<0.05).Conclusion: Low-power gallium aluminium arsenide laser irradiation on open skin wound of rats reduced significantly total number of mast cells and intact ones at inflammatory phase, and 90 significantly increased active mast cells at proliferation, and intact ones at remodeling phases of the wound healing process.
M Bayat; SN Razavi; A Hosseini
Volume 4, Issue 2 , 2001, , Pages 3-8
Abstract
Background: Positive effects of Kombucha tea (An old folkloric drink of Asian people) on wound healing process have been observed. Objective: To study its effects on the number of mast cells of wound bed. Materials and Methods: The study design was experimental. 42 male adult rats were randomly divided ...
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Background: Positive effects of Kombucha tea (An old folkloric drink of Asian people) on wound healing process have been observed. Objective: To study its effects on the number of mast cells of wound bed. Materials and Methods: The study design was experimental. 42 male adult rats were randomly divided into control and experimental groups. Each group was divided into 3 subgroups. The time of study of first subgroups were 4 days (Represents inflammation phase of wound healing process), of second subgroups were 7 days (Proliferation phase) and of third subgroups were 15 days (Remodeling phase) after wounding. Both groups drank Kombucha tea for 30 days. Then under general anesthesia and sterile conditions, a full thickness wound was made on the dorsum of all rats. The wounding day was considered as day zero. Rats of experimental group drank Kombucha tea after surgery until they were killed by ether at the end of above-mentioned durations. Samples were obtained from rats and prepared for light microscopical study and stained with 1% watery solution of toluidine blue. Mast cells and its grades (According to it’s degranulations) were counted. Data were analyzed by students t test method. Results: Main results were: 1) Total number of most cells of experimental group decreased from 7th day toward 15th day and on 15th day, the difference with control group was significant (P<0.01); 2) In most cases the numbers of mast cells of experimental group were lower than control group and the difference were significant for the 4th day grade 1, 15th day grade 2 (P<0.05) and total number of grades 2 and 3 (P<0.01). Conclusion: Daily consumption of Kombucha tea by rats caused a decreased number of mast cells during skin wound healing and the difference with control group at remodeling phase was significant.