Lotfollah Afzali; Shima Rahmati; Reza Zargarsaleh; Babak Alavi-Farzaneh; Mehdi Banitalebi
Abstract
Background: Wound healing in grade II burns is one of the treatment priorities. Advances in new sciences, such as stem cell therapy, biological scaffolds, and growth factors, have renewed hopes in this area. The present study aimed to assess the impact of ADSCs encapsulated in PRP/Chitosan-based gel ...
Read More
Background: Wound healing in grade II burns is one of the treatment priorities. Advances in new sciences, such as stem cell therapy, biological scaffolds, and growth factors, have renewed hopes in this area. The present study aimed to assess the impact of ADSCs encapsulated in PRP/Chitosan-based gel and PRP/Chitosan cell-free gel on the healing of grade-II burn wounds in rats as compared to the control group.Methods: Thirty rats were randomly assigned to one of three groups. Following adipose stem cell extraction, a deep grade II burn was induced in the back of the rats using a standardized catheter. The first group served as a control and received simply routine treatments. The second group received a gel based on PRP/Chitosan and ADSCs, and the third group received a gel free of PRP/Chitosan cells. The results were based on response to treatment, observable granulation tissue formation, or epithelialization at 7, 14, 21, and 28 days after treatment.Results: The findings revealed that the use of a cell-based PRP/ Chitosan scaffold or cell-free PRP/Chitosan scaffold reduced the rate of necrosis and inflammation and increased the rate of epithelialization, granulation, and neovascularization compared to the control group (P < 0.05). Moreover, the use of stem cells in scaffolds resulted in greater wound healing than the cell-free scaffolds group (P < 0.05).Conclusion: Due to their porosity and the improved efficacy of stem cells placed in them, PRP/Chitosan scaffolds could have a positive impact on healing and speed up the wound healing process.
Nurona Azizah; Musthika Mashitah; Heri Kristianto
Abstract
Background: The management of third-degree burns primarily aims to enhance the re-epithelialization process. Any impairment in this process may cause delayed wound healing. Rosella calyx (Hibiscus sabdariffa L.) can act as an immunomodulator, possibly affecting the re-epithelialization process.Methods: ...
Read More
Background: The management of third-degree burns primarily aims to enhance the re-epithelialization process. Any impairment in this process may cause delayed wound healing. Rosella calyx (Hibiscus sabdariffa L.) can act as an immunomodulator, possibly affecting the re-epithelialization process.Methods: Rats were randomized after adaptation for a week. Rats (n = 30) were induced with third-degree burns, and it was ensured that they met the criteria for third-degree burns. We divided them into six groups and administered treatment according to each group: 1) negative control group using cold cream only; 2) positive control using silver sulfadiazine (SSD) only; 3) treatment group using rosella calyx extract orally (250 mg/kg) + cold cream; 4) treatment group using rosella calyx extract topically only (250 mg/kg); 5) treatment group using rosella calyx extract orally (250 mg/kg) + SSD; and 6) treatment group using rosella calyx extract orally and topically (each 250 mg/kg). They were sacrificed on day 17 and then stained with hematoxylin and eosin. The variable studied was the re-epithelialization length (mm). Statistical analysis involved analysis of variance (ANOVA) and post hoc correction with P < 0.05 taken as significant.Results: Despite treatment using rosella calyx extract orally + topically showing the longest re-epithelialization, this group showed no significant difference compared with SSD treatment, indicating they may show a similar effect in the re-epithelialization process.Conclusion: Rosella calyx extract applied orally and topically may show a therapeutic effect in the re-epithelialization process. Further research needs to be conducted.
