Document Type : Original Article
Authors
- Jannan Ghapanchi 1
- Maryam Zahed 1
- Mohammad Javad Mokhtari 2
- Mohammad Javad Fattahi 3
- Fahimeh Rezazadeh 1
- Nafiseh Moshtaghi 4
1 Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran
3 Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Background: This study aimed to assess the single nucleotide polymorphisms (SNPs) of lncRNA (long non-coding RNA) H19 rs217727 in patients with oral lichen planus (OLP) compared to controls.
Methods: We collected 270 DNA samples of OLP cases and healthy individuals. We used the ARMS-PCR tetra primer for DNA genotyping and applied specific primer pairs.
Results: The prevalence of the rs217727 C allele was lower in OLP cases than in healthy subjects (P = 0.005). The prevalence of TT genotypes of H19 rs217727 was greater in OLP patients compared with healthy subjects (5.71% vs 1.5%). Also, the TT genotype in the codominant model was associated with a 5.15-fold higher risk of OLP (P = 0.02). In the dominant model, the CT+TT genotypes were associated with a 2.09-fold greater risk of OLP (P = 0.007). The H19 rs217727 polymorphism was linked to a 3.95-fold greater risk of OLP in the recessive model (P = 0.05) (TT vs. CC+CT).
Also, in the over-dominant model, the CT genotypes were related to a 1.78-fold greater risk of OLP (P = 0.03).
Conclusion: This study demonstrated a significant link between lncRNA H19 polymorphism and OLP lesions. Further studies on larger populations are necessary to confirm this relationship.
Keywords
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